Rapid detection of methicillin resistance in <Emphasis Type="Italic">Staphylococcus aureus</Emphasis> isolates; evaluation of colorimetric Quicolor ES agar and determination of breakpoint inhibition zone diameters of cefoxitin |
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| Authors: | Ahmet Yilmaz Coban Ugur Dem?rpek Tuba Y?ld?r?m Yeliz Tanr?verd? Cayc? Tanil Kocagoz Belma Durup?nar |
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| Institution: | (1) Medical School, Department of Medical Microbiology, Ondokuz Mayis University, 55139 Samsun, Turkey;(2) Kyrenia Hospital, Clinical Microbiology Laboratory, Kyrenia, Cyprus;(3) Faculty of Arts and Science, Department of Biology, Amasya University, Amasya, Turkey;(4) Medical School, Department of Medical Microbiology, Acibadem University, Maltepe, Istanbul, Turkey |
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| Abstract: | In this study, it was aimed to evaluate colorimetric Quicolor ES agar for the rapid detection of methicillin resistance and
to determine susceptibility and resistance breakpoint zone diameters for cefoxitin by using 51 methicillin susceptible Staphylococcus aureus (MSSA) and 63 methicillin resistant S. aureus (MRSA) isolates. In the study, while oxacillin and cefoxitin results were obtained within 4–7 h (5.5 h in average) for MSSA
isolates, the results of MRSA isolates were obtained within 5.5–9 h (6.6 h in average) for both antibiotics on QC ES agar.
QC ES agar is an inexpensive medium for rapid detection (4–9 h) of methicillin resistance by disc diffusion method using oxacillin
or cefoxitin. Additional studies for further evaluation of the efficiency of QC-ES agar in rapid determination of methicillin
resistance in S. aureus may be beneficial. |
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