| The cDNA and Genomic DNA Organization of a Novel Toxin SHT-I from Spider Ornithoctonus huwena |
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| 作者姓名： | Qiao P Zuo XP Chai ZF Ji YH |
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| 作者单位： | Peng QIAO，Xiao-Pan ZUO，Zhi-Fang CHAI，and Yong-Hua JI* Institute of Physiology，Shanghai Institutes for Biological Sciences，Chinese Academy of Sciences，Graduate School of the Chinese Academy of Sciences，Shanghai 200031，China |
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| 基金项目： | The work was supported by the grants from the National Basic
Research Program of China (1999054001)，and partially the National Nature Science Foundation of China (30270428) |
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| 摘 要： | Spider venoms are complex mixtures of neurotoxicpeptides, proteins and low molecular mass organicmolecules. Their neurotoxic activity is due to the interac-tion of the venom components with cellular receptors, inparticular ion channels. Spider venoms have…
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| 关 键 词： | 毒素 脱氧核糖核酸 蜘蛛 染色体组 肽 |
The cDNA and genomic DNA organization of a novel toxin SHT-I from spider Ornithoctonus huwena |
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| Qiao P,Zuo XP,Chai ZF,Ji YH.The cDNA and Genomic DNA Organization of a Novel Toxin SHT-I from Spider Ornithoctonus huwena[J].Acta Biochimica et Biophysica Sinica,2004,36(10):656-660. |
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| Authors: | Qiao Peng Zuo Xiao-Pan Chai Zhi-Fang Ji Yong-Hua |
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| Institution: | Institute of Physiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China. |
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| Abstract: | In present study the full-length cDNA of a novel toxin from the venom gland of spider Ornithoctonus huwena, named as SHT-I, has been cloned using the strategy of rapid amplification of cDNA ends, and then the whole genomic sequence of SHT-I (Selenocosmia huwena toxin-I) was determined using sequence-specific primers synthesized based on the acquired 3' and 5' ends of SHT-I cDNA sequence. It is unexpectedly found that intron was lacking in the genomic sequence of SHT-I. The result might evoke an interesting question whether the gene code of inhibitor cystine-knot peptides from spider venom is distinct from that of those known toxic peptides of scorpion and cone snail. |
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| Keywords: | Ornithoctonus huwena spider cDNA genomic DNA peptide toxin ICK motif |
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