Effect of natural ageing and antioxidant inhibition on liver antioxidant enzymes,glutathione system,peroxidation, and oxygen consumption inRana perezi

Summary A study of the physiological role of oxygen free radicals in relation to the ageing process was performed using the liver ofRana perezi, an animal with a moderate rate of oxygen consumption and a life span substantially longer than that of laboratory rodents.Among the five different antioxidant enzymes only superoxide dismutase (SOD) showed an age-dependent decrease. Cytochrome oxidase (COX), glutathione status, in vivo and in vitro liver peroxidation, and metabolic rate did not vary as a function of age.Long-term (2.5 months) treatment with aminotriazole and diethyldithiocarbamate depleted catalase (CAT) activity and did not change both glutathione peroxidases (GPx), COX, reduced (GSH) and oxidized (GSSG) glutathione, or metabolic rate. This treatment resulted in great compensatory increases in SOD (to 250–460% of controls) and glutathione reductase (GR) (to 200%) which are possibly responsible for the lack of increase of in vivo and in vitro liver peroxidation and for the absence of changes in survival rate.The comparison of these results with previous data from other species suggests the possibility that decreases in antioxidant capacity in old age are restricted to animal species with high metabolic rates. Nevertheless, ageing can still be due to the continuous presence of small concentrations of O₂ radicals in the tissues throughout life in animals with either high or low metabolic rates, because radical scavenging can not be 100% effective. Compensatory homeostasis among antioxidants seems to be a general phenomenon in different species.Abbreviations AT 3-amino-1,2,4 triazole - CAT catalase - COX cytochrome c oxidase - DDC diethyldithiocarbamate - GPx glutathione peroxidase - GR glutathione reductase - GSH reduced glutathione - GSSG oxidized glutathione - MDA malondialdehyde - SOD superoxide dismutase - TBA-RS thiobarbituric acid-reacting substances - VO ₂ oxygen consumption