Quantitative carbon-14 and tritium assay of thin-layer chromatography plates |
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| Affiliation: | 1. Department of Chemistry, University of Helsinki, P.O. Box 55, 00014, Helsinki, Finland;2. Nagra (National Cooperative for the Disposal of Radioactive Waste), Wettingen, Switzerland;3. Posiva Oy, Finland;1. Graduate School of Science, Osaka University, Suita, Osaka 565-0871, Japan;2. JST, ERATO, Lipid Active Structure Project, Osaka 565-0871, Japan;3. Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan;4. Faculty of Pharmacy, Chiba Institute of Science, Choshi, Chiba 288-0025, Japan;5. Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan;6. SOSHO Inc., Suita, Osaka 565-0871, Japan;7. Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto 606-8522, Japan;8. Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa 226-8501, Japan;9. Amine Pharma Research Institute, Innovation Plaza at Chiba University, Chuo-ku, Chiba 260-0856, Japan;10. Department of Biotechnology, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan;1. Department of of Phthisiology and Pulmonology, Kharkiv National Medical University, 4 Lenina Avenue, Kharkiv 61022, Ukraine;2. Department of Pathomorphology, Kharkiv National Medical University, 4 Lenina Avenue, Kharkiv 61022, Ukraine;3. Department of Internal Medicine N3, Kharkiv National Medical University, 4 Lenina Avenue, Kharkiv 61022, Ukraine |
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| Abstract: | A fast and reliable technique for recovering essentially 100% of total carbon-14- and tritium-labeled lipids separated on thin-layer chromatography (TLC) plates is described. Cab-O-Sil, a gelling agent, was used to suspend the TLC adsorbent in a liquid scintillator solution, and for this reason the procedure requires no elution steps. |
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