The ontogeny of isozyme patterns of lactate dehydrogenase in the mouse |
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| Affiliation: | 1. Department of Biomedical Sciences, Faculty of Veterinary Medicine, University of Prince Edward Island, 550 University Ave., Charlottetown, PEI, C1A 4P3, Canada;2. AVC Lobster Science Centre, Faculty of Veterinary Medicine, University of Prince Edward Island, 550 University Ave., Charlottetown, PEI, C1A 4P3, Canada;3. Department of Plant and Animal Sciences, Faculty of Agriculture, Dalhousie University, PO Box 500, Truro, Nova Scotia, B2N 5E3, Canada;1. Department of Surgery, University of Maryland, Baltimore, MD;2. National Study Center for Trauma & EMS, School of Medicine, University of Maryland, Baltimore, MD;3. Department of Surgery, R Adams Cowley Shock Trauma Center & University of Maryland, Baltimore, MD;4. Department of Diagnostic Radiology & Nuclear Medicine, R Adams Cowley Shock Trauma Center & University of Maryland, Baltimore, MD;1. IPHES, Institut Català de Paleoecologia Humana i Evolució Social, Campus Sescelades URV (Edifici W3), 43007 Tarragona, Spain;2. Àrea de Prehistòria, Universitat Rovira i Virgili (URV), Av. Catalunya 35, 43002 Tarragona, Spain;3. ICREA, Institució Catalana de Recerca i Estudis Avançats, Barcelona, Catalonia, Spain;1. Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, Taiwan;2. Department of Aquaculture, National Kaohsiung Marine University, Kaohsiung, Taiwan;3. College of Aquaculture and Fisheries, CanTho University, CanTho, Vietnam;4. Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan;1. Department of Food Science and Technology, Faculty of Agriculture, University of Khartoum, Shambat 13314, Sudan;2. Department of Agricultural, Biological and Environmental Sciences, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan;3. Laboratory of Plant Genetics, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan;4. Arid Land Research Center, Tottori University, 1390 Hamasaka, Tottori 680-0001, Japan |
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| Abstract: | The enzyme lactate dehydrogenase (LDH) was shown to exist as five electrophoretically distinct molecular varieties, or isozymes, within the tissues of the mouse.Nearly every tissue or organ of the adult mouse contains all five isozymes of LDH, but in proportions that are highly specific for the tissue.The pattern of isozyme distribution in embryonic tissues differs from the adult pattern. Most embryonic tissues initially contain principally LDH-5; as development proceeds LDH activity is gradually transposed toward the LDH-1 end of the spectrum.The LDH isozymes differ in kinetic properties, e.g., substrate concentration optima; these differences apparently enable the isozymes to fulfill distinct metabolic roles in cellular metabolism. LDH-5 is more abundant in tissues subject to relative anaerobiosis, and the isozymes at the other end of the spectrum are more abundant in highly oxygenated tissues.The isozyme patterns in the mouse are satisfactorily explained by the previously elaborated subunit hypothesis (Markert, 1962), which pictures the LDH molecule as composed of four polypeptide subunits that may be separated into two distinct varieties, A and B, each presumably under the control of a separate gene. Assortment of these subunits in all possible combinations of four generates the five isozymes of LDH. The relative proportions of A and B synthesized by a cell would determine the tissue-specific patterns of LDH isozymes either in the adult or during the course of embryonic or neonatal development. |
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