褪黑素受体激动剂改善高糖高脂喂养大鼠脂联素敏感性

目的观察褪黑素受体激动剂（NEU-P11）对高糖高脂饲养大鼠脂联素敏感性的影响。方法将30只SD大鼠随机分为对照组（CD组），高糖高脂组（HFSD组），褪黑素组（Mel组），褪黑素受体激动剂组（NEU-P11组）。CD组饲以正常饲料；其余3组饲以高糖高脂饲料。6个月后，给药治疗2个月。治疗期间，Mel组每天注射Mel（4mg／kg）；NEU—P11组每天注射NEU-P11（10mg／kg）；CD组以及HFSD组注射生理盐水（5ml／kg）。测定糖脂代谢指标并做口服葡萄糖耐量实验（oral glucose tolerant test，OG-TY），Western印迹检测脂联素（adiponectin，APN）在脂肪组织及脂联素受体（AdipoR）在骨骼肌组织中的表达变化。结果高糖高脂饮食可诱导SD大鼠产生胰岛素抵抗，脂联素表达增加。Neu-P11治疗后，胰岛素敏感性增强．脂联素表达降低至正常水平。结论Neu-P11能提高胰岛素敏感性，改善脂联素抵抗。

Effect of NEU-Pll on Adiponectin Sensitivity of High-Sucrose/High- Fat Fed SD Rats

Objective To investigate the effect of Neu-P11 on Adiponectin sensitivity in Sprague Dawley （SD） rats fed by high-sucrose/high-fat diets （HFSD） . Methods 30 male SD rats were randomly assigned to four dietary groups： normal control diet group （CD） , fed with standard diet; high fat and high sucrose diet group （HFSD）, Melatonin group （Mel） and NEU-P11 group （NEU-Pll）, fed with 53% normal diet supplemented with 10% lard and 37% sucrose. All rats were fed for 6 months and treated for 2 months. During drug administration, normal control group and HFSD group were treated with intraperitoneal injection of physiological saline （5ml/kg） , while NEU-P11 treated hyperglycemic group and Mel treated hyperglycemic group were treated with intraperitoneal injection of melatonin receptor agonist （10mg,/kg） and melatonin （4mg/kg） respectively. At the end of every month the oral glucose tolerant test （OGTT） were performed ; Adiponeetin and Adiponectin receptor （AdipoR） were detected by western-blot. Results In HFSD group fed with high fat/high sucrose diet, insulin resistance was induced with increase of Adiponectin expression. After administration of NEU-P11, insulin sensitivity was improved with decrease of Adiponectin expression. Conclusion NEU-P11 can enhance insulin sensitivity, thereby reducing adiponectin resistance of insulin-resistant rats.