3个玉兰材料对水涝胁迫的响应与耐涝性评价

为评价星花玉兰及其实生选育品种‘菊花’以及其与柳叶玉兰的杂交品种‘皮鲁埃特’的耐涝性强弱,并为后续星花玉兰耐涝品种选育提供理论依据。通过14 d的水涝胁迫,研究3种玉兰叶片生理、内源激素和光合特性等相关的14个指标变化。利用主成分分析法,比较超氧化物歧化酶(SOD)、丙二醛(MDA)、可溶性蛋白、脱落酸(ABA)和1-氨基环丙烷-1-羧酸(ACC)这5个作为评价耐涝性指标的重要性以及评价耐涝性,同时利用隶属函数分析法对5个指标进行耐涝性评价。结果表明:(1)3种玉兰在水涝胁迫后叶片的净光合速率(P n)、气孔导度(G_(s))、蒸腾速率(T_(r))、叶绿素含量、初始荧光(F_(o))、最大荧光(F_(m))、PSⅡ最大光化学量子产量(F_(v)/F_(m))、PSⅡ有效光化学量子产量(Yield)和可溶性蛋白含量均显著降低,MDA、ABA含量和SOD活性均有不同的增加幅度。‘菊花’和星花玉兰处理组的C_(i)、ACC显著增加,而‘皮鲁埃特’的显著降低。(2)评价3种玉兰耐涝性的生理及内源激素指标重要性依次为ACC、SOD、ABA、可溶性蛋白和MDA。(3)通过主成分分析法和隶属函数分析法对3种玉兰进行综合评价,耐涝性强弱依次为‘皮鲁埃特’、星花玉兰和‘菊花’。

Response to Waterlogging Stress and Evaluation of Waterlogging Tolerance of Three Yulania Materials

To evaluate the waterlogging tolerance of Y. stellata and its seedling breeding cultivars Y. stellata ''Chrysanthemiflora'' and its hybrid cultivars Y. ''Piroutte'' with Y. salicifolia, and provide theoretical basis for subsequent selection of Y. stellata waterlogging tolerant cultivars. Through 14 days of waterlogging stress, the changes of 14 indexes related to leaf physiology, endogenous hormones and photosynthetic characteristics of three Yulania denudata were studied. Using principal component analysis, the importance of superoxide dismutase (SOD), malondialdehyde (MDA), soluble protein, abscisic acid (ABA) and 1-aminocyclopropane-1-carboxylic acid (ACC) as indicators of waterlogging tolerance was compared and waterlogging tolerance was evaluated. The waterlogging tolerance of the five indicators was evaluated by the membership function analysis method. The results showed that: (1) The contents of Pn, Gs, Tr, chlorophyll, Origin fluorescence, Maximum fluorescence, PSII maximum photochemical quantum yield, PSII effective photochemical quantum yield and soluble protein in the leaves of the three Yulania denudata were significantly decreased after waterlogging stress, and the contents of MDA, ABA and SOD activities were increased by different degrees. However, the Intercellular CO2 concentration and ACC of Y. stellata ''Chrysanthemiflora'' and Y. stellata treatment groups increased significantly, while the Ci and ACC of Y. ''Piroutte'' decreased significantly. (2)The importance of physiological and endogenous hormone indicators to evaluate the waterlogging tolerance of three Yulania denudata is in order: ACC, SOD, ABA, soluble protein, and MDA. (3) According to the comprehensive evaluation of the three plants by principal component analysis and membership function analysis, the waterlogging tolerance of the three Yulania denudata is in the order of Y. ''Piroutte'', Y. stellata and'' Y. stellata ''Chrysanthemiflora''.