<Emphasis Type="Italic">In vitro</Emphasis> stem elongation of stemless carline thistle |
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Authors: | Grubišić Dragoljub Katarina Šavikin-Fodulović Danijela Mišić Giba Zlatko Konjević Radomir |
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Institution: | (1) Institute for Biological Research, S. Stankovi, 29. novembra 142, Yugoslavia;(2) Institute for Medicinal Plant Research, Tadeua Kouka 1, Yugoslavia;(3) Institute of Botany, Faculty of Biology, University of Belgrade, Takovska 43, Yugoslavia |
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Abstract: | In vitro culture of stemless carline thistle was established using immature zygotic embryos. A satisfactory bud multiplication was achieved on MS medium supplemented with BAP (1 mg L–1) and IAA (0.2 mg L–1). Maximum rooting of buds was induced upon short cultivation (4 or 8 days) on an auxin-supplemented medium. Highest number of roots was obtained with NAA in the medium while the longest roots developed on the IAA-supplemented medium. Plantlets that subsequently developed were in rosette form if grown in light (16/8 h light to darkness photoperiod) and with elongated stems if raised in darkness. Light grown plantlets treated with GA3 showed dose dependent stem increase in length, reaching maximum at the concentration of 10–4 M. This was correlated with the length and the average number of internodes. If cultivated in the presence of ancymidol, dark grown plantlets showed reduced stem length. However, the inhibitory effect of the growth retardant on stem elongation was completely overcome by the addition of GA3. |
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Keywords: | Gibberellins Light Retardants Stem elongation |
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