Composition of Caulobacter crescentus murein synthesized in the presence of glycine |
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Authors: | Zdzislaw Markiewicz Werner Kraus |
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Institution: | Institute of Microbiology, University of Warsaw, Nowy Swiat 67, 00-046 Warsaw, Poland;Max-Planck-Institut für Entwicklungsbiologie, Spemannstrasse 35, D-7400 Tübinger, F.R.G. |
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Abstract: | Abstract Glycine added to the growth medium of Caulobacter crescentus was found to substitute Cterminal alanine in the peptide side chains of the murein of this species. Murein synthesized in vivo and in vitro in the presence of glycerine was poorly crosslinked as was new murein formed in the presence of the amino acid. The reduced cross-linkage seems to be due to the effect of glycine on the formation of trimeric muropeptides as revealed by high-performance liquid chromatography (HPLC) muropeptide analysis of murein formed in the presence and absence of the amino acid. |
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Keywords: | Caulobacter crescentus murein composition effect of glycine on murein |
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