Adiponectin gene polymorphisms (T45G and G276T), adiponectin levels and risk for metabolic diseases in an Arab population |
| |
Authors: | Al-Daghri Nasser M Al-Attas Omar S Alokail Majed S Alkharfy Khalid M Hussain Tajamul Yakout Sobhy Vinodson Benjamin Sabico Shaun |
| |
Institution: | a Biomarkers Research Program, Department of Biochemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabiab Center of Excellence in Biotechnology Research, King Saud University, Riyadh 11451, Saudi Arabiac Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia |
| |
Abstract: | In this study we examined the association of adiponectin gene variants with circulating adiponectin, and known metabolic diseases in 298 healthy controls and 297 Saudi subjects with type 2 diabetes mellitus (T2DM). Anthropometric and biochemical parameters were measured by standard procedures. Genotyping of T45G and G276T single nucleotide polymorphisms of adiponectin gene was carried out by PCR-RFLP analysis. No significant differences in the genotype distribution of T45G and G276T polymorphism were found between control and diabetic subjects. Neither SNP conferred an association with T2DM, obesity, hypertension or dyslipidemia. Despite a marked decrease in patients as opposed to controls, adiponectin levels were not different according to genotypes of T45G and G276T polymorphisms in control and patients. Thus, neither adiponectin SNPs independently conferred increased T2DM risk nor in other metabolic conditions considered such as obesity, hypertension or dyslipidemia. These findings support the existence of population based differences in the association of adiponectin gene variants with metabolic phenotypes and emphasize the importance of studying multiple polymorphisms, sufficient enough to identify the adiponectin gene as a genetic marker for several non-chronic communicable diseases. |
| |
Keywords: | BMI body mass index DNA deoxyribonucleic acid HDL-Cholesterol high density lipoprotein cholesterol HOMA-IR homeostasis model assessment for insulin resistance PCR polymerase chain reaction PPAR peroxisome proliferator activated receptor RFLP restriction fragment length polymorphism SNP single nucleotide polymorphism SPSS statistical Package for the Social Sciences T2DM type 2 Diabetes Mellitus |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|