The IsdG‐family of haem oxygenases degrades haem to a novel chromophore |
| |
Authors: | Michelle L Reniere Georgia N Ukpabi S Reese Harry Donald F Stec Robert Krull David W Wright Brian O Bachmann Michael E Murphy Eric P Skaar |
| |
Institution: | 1. Departments of Microbiology and Immunology and;2. Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.;3. Chemistry, Vanderbilt University Medical Center, Nashville, TN, USA.;4. Bruker Biospin, Billerica, MA, USA. |
| |
Abstract: | Enzymatic haem catabolism by haem oxygenases is conserved from bacteria to humans and proceeds through a common mechanism leading to the formation of iron, carbon monoxide and biliverdin. The first members of a novel class of haem oxygenases were recently identified in Staphylococcus aureus (IsdG and IsdI) and were termed the IsdG‐family of haem oxygenases. Enzymes of the IsdG‐family form tertiary structures distinct from those of the canonical haem oxygenase family, suggesting that IsdG‐family members degrade haem via a unique reaction mechanism. Herein we report that the IsdG‐family of haem oxygenases degrade haem to the oxo‐bilirubin chromophore staphylobilin. We also present the crystal structure of haem‐bound IsdI in which haem ruffling and constrained binding of oxygen is consistent with cleavage of the porphyrin ring at the β‐ or δ‐meso carbons. Combined, these data establish that the IsdG‐family of haem oxygenases degrades haem to a novel chromophore distinct from biliverdin. |
| |
Keywords: | |
|
|