Hyperosmosis enhances radiation and hydroxyurea resistance of Schizosaccharomyces pombe checkpoint mutants through the spindle checkpoint and delayed cytokinesis |
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| Authors: | John P Alao Pim J Huis in 't Veld Frederike Buhse Per Sunnerhagen |
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| Institution: | 1. Department of Cell and Molecular Biology, Lundberg Laboratory, University of Gothenburg, P.O. Box 462, S‐405 30 G?teborg, Sweden.;2. Present address: Research Institute of Molecular Pathology, Dr Bohr‐Gasse 7, A‐1030 Vienna, Austria. |
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| Abstract: | The DNA damage and stress response pathways interact to regulate cellular responses to genotoxins and environmental stresses. How these pathways interact in Schizosaccharomyces pombe is not well understood. We demonstrate that osmotic stress suppresses the DNA damage sensitivity of checkpoint mutants, and that this occurs through three distinct cell cycle delays. A delay in G2/M is dependent on Srk1. Progression through mitosis is halted by the Mad2‐dependent spindle checkpoint. Finally, cytokinesis is impaired by modulating Cdc25 expression. These three delays, imposed by osmotic stress, together compensate for the loss of checkpoint signalling. |
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