湿氧化爆破稻秆的同步糖化发酵条件研究

探讨了木质纤维素经过湿氧化爆破后在同步糖化发酵过程中酵母产乙醇的基本规律.采用单因素方法对湿氧化爆破条件、酶系组成和添加量以及预酶解时间和温度进行了优化.不同湿氧化爆破预处理条件下的稻秆对同步糖化发酵工艺的影响较大,在预处理温度160 ℃,进氧压力为4×105 Pa,碱用量为6%(w/w),反应时间为20 min的条件...

Process of simultaneous saccharification and fermentation for rice straw after wet-oxidation explosion

The regularity of the simultaneous saccharification and fermentation of yeast for the production of ethanol was studied. The effects of the conditions of wet explosion, the anqount of ceUulase, β-gluccsidase, xylanase and the pre-enzymatic hydrolysis time on the simultaneous saccharification and fernaentation were studied by single factor merle. The rcsults showed that the wet oxidation explosion for the rice straw under different conditions had obvious effects. The pretreated feedstock at 160℃, the inlet pressure 4 ×10^5Pa, the alkalic amount 6% （w/w）, the reaction time 20 min eould improve the saccharificafion and fermentation efficiency. The optimal condifions obtained through the simultaneous saccharificafion and fermentation （SSF） were as follows： the cellulase 20 IU/g cellulose, β-gluoosidase 40 IU/g eellulose, xylanase 800 IU/g at 4512 for 8 h prermyrnatic hydrolysis and 72 h SSF hydrolysis. The best eonversion of ethanol with 85 % was obtained, based on the dry mass of cellulose. The conversion field of ethanol was up to 70% on the substrate 100 g/L cellulose. The method of the simultaneous saccharification and fermentation for the production of ethanol using the wet-oxidation exploded rice straw was feasible for the industrial prospect.