| 用染色体原位杂交技术定位RFLP探针Fr.3-42于16p13 |
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| 引用本文： | 萧广惠.用染色体原位杂交技术定位RFLP探针Fr.3-42于16p13[J].遗传学报,1990(5). |
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| 作者姓名： | 萧广惠 |
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| 作者单位： | 湖南医科大学附属第一医院中心实验室 长沙 |
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| 摘 要： | 限制性片段长度多态性(RFLP)探针Fr.3-42(第九届人类基因定位国际会议编号D1(?)S21)为一长1.9kb的人类单拷贝EcoRI/HindⅢ片段,本实验采用染色体原位杂交方法,将该探针定位于16号染色体短臂末端(p13)。在另一研究中已证实Fr.3-42与人α-珠蛋白基因紧密连锁。
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| 关 键 词： | 原位杂交 16号染色体 RFLP探针 |
Localization of RFLP Probe Fr. 3-42 at 16p 13 Band by in situ Hybridization |
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| Xiao Guanghui.Localization of RFLP Probe Fr. 3-42 at 16p 13 Band by in situ Hybridization[J].Journal of Genetics and Genomics,1990(5). |
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| Authors: | Xiao Guanghui |
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| Abstract: | Recombinant plasmid clone Fr. 3-42(HGM9 no. D16S21) containing a human single-copy DNA segment of 1.9kb was hybridized to human chromosome preparations. Twenty-eight percent of hybridized metaphases exhibited silver grains at the terminal region of short arm of chromosome 16 (16pl3). Close linkage between this probe and the human alpha 1 hemoglobin gene was demonstrated in another study. |
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| Keywords: | in situ hybridization chromosome 16 RFLP probe |
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