Intergeneric protoplast fusion in lactic acid bacteria |
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Authors: | PS Cocconcelli L Morelli M Vescovo V Bottazzi |
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Institution: | Research Institute ITAL, P.O. Box 48, 6700 AA Wageningen, The Netherlands |
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Abstract: | Abstract Crystals from Bacillus thuringiensis var. israelensis appeared to contain three major proteins of M r 230 000, 130 000 and 28 000. These proteins were solubilized from the crystals by incubation in 10 mM DTT, pH 9.5, and purified by sucrose gradient centrifugation. The M r 230 000 and 130 000 crystal proteins showed mosquitocidal properties, whereas the M r 28 000 crystal protein contained haemolytic activity. Immobilization of these proteins on latex beads did not alter these properties. Partial proteolytic degradation showed that the M r 130 000 and 28 000 proteins are structurally different. |
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Keywords: | Crystal solubilization sucrose gradient fractionation haemolytic activity |
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