| 用固定化方法研究超氧化物歧化酶的活性与结构的关系 |
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| 引用本文： | 袁勤生,邓碧玉,杨曜中,李文杰.用固定化方法研究超氧化物歧化酶的活性与结构的关系[J].中国生物化学与分子生物学报,1992,8(3):326-330. |
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| 作者姓名： | 袁勤生 邓碧玉 杨曜中 李文杰 |
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| 作者单位： | 华东化工学院，华东化工学院，华东化工学院，中科院上海生物化学研究所 上海 200237，上海 200237，上海 200237 |
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| 摘 要： | 采用吸附、包埋、共价交联等方法固定化超氧化物歧化酶(SOD)所得固定化酶活力回收都不高,表明酶的催化反应速率受超氧阴离子自由基(O_2~-)扩散速率的控制。用海藻酸钠包埋SOD,固定化酶不表现活力,破固定化后所得的糊状物却有很高的活力。用戊二醛交联所得的固定化酶活力回收率也很低,表明ε-NH_3~+的正电荷是酶活力所必需。
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| 关 键 词： | 铜锌超氧化物歧化酶 固定化方法 催化作用机理 |
| 收稿时间： | 1992-06-20 |
Studies on the Mechanism of Action of Porcine Cu-Zn-SOD by Different Methods of Immbilization of this Enzyme |
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| Yuan,Qin-sheng Deng,Bi-yu Yang,Yao-zhong Li,Wen-jie.Studies on the Mechanism of Action of Porcine Cu-Zn-SOD by Different Methods of Immbilization of this Enzyme[J].Chinese Journal of Biochemistry and Molecular Biology,1992,8(3):326-330. |
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| Authors: | Yuan Qin-sheng Deng Bi-yu Yang Yao-zhong Li Wen-jie |
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| Institution: | (East China Institute of Chemical Technology,Shanghai 200237 |
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| Abstract: | Immobilized SOD were obtained by absorption, entrapment and covalent cross-linking, and all the immobilized enzyme activities were low.It appears that reaction rate of the copper and zinc containing superoxide dismutase with superoxide anion is near the limit of diffusion control.Using the sodium aiginate entrapping method, the immobilized Cu***********..Zn-SOD does not show any activity, after dissolving the immobilized enzyme in phophate buffer, the enzyme regains its activity. |
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| Keywords: | Cu·Zn-SOD Immobilized methods |
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