Selection of auxotrophic and carbohydrate-negative mutants in penicillin-resistant Klebsiella pneumoniae by nalidixic acid treatment |
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| Authors: | Georg A. Sprenger Alfried Vogler Joseph W. Lengeler |
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| Affiliation: | Fachbereich Biologie/Chemie, Arbeitsgruppe Genetik, Universität Osnabrück, Postfach 4469 D-4500 Osnabrück, F.R.G. |
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| Abstract: | Abstract The bactericidal antibiotic nalidixic acid can be used to select in cultures of Klebsiella pneumoniae and other penicillin-resistant enteric bacteria any auxotrophic and carbohydrate-negative mutant. Nalidixic acid treatment is superior to penicillin or d -cycloserine treatment, since no cross-feeding by lysed cells is possible, and it is simple to handle. |
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| Keywords: | Klebsiella pneumoniae nalidixic acid mutant selection anxotrophic mutant carbohydrate-negative mutant |
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