Convenient and rapid analysis of linkage isomers of fucose-containing oligosaccharides by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry |
| |
Authors: | Yusuke Suzuki Minoru Suzuki Emi Ito Hiroshi Ishii Kouzou Miseki Akemi Suzuki |
| |
Institution: | (1) Sphingolipid Expression Laboratory, Supra Biomolecular System Research Group, RIKEN Frontier Research System, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan;(2) Shimadzu Corporation, 1 Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan |
| |
Abstract: | Matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) was used
to analyze three pyridylamino (PA)-fucosyloligosaccharides isolated from human milk: lacto-N-fucopentaose (LNFP) I Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1-4Glc-PA],
LNFP II Galβ1-3(Fucα1-4)GlcNAcβ1-3Galβ1-4Glc-PA], and LNFP III Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ1-4Glc-PA]. These oligosaccharides
are linkage isomers. MALDI-QIT-TOF MS provides MSn spectra, which we used to characterize these PA-oligosaccharides. MS/MS/MS analysis of the non-reducing end tri-saccharide
ions generated by MS/MS was able to distinguish these oligosaccharide isomers. The MALDI-QIT-TOF MS is a very convenient and
rapid method, therefore, it would be useful for high throughput structural analyses of various types of pyridylaminated oligosaccharide
isomers. |
| |
Keywords: | MALDI mass spectrometry PA sugars lactofucopentaoses |
本文献已被 PubMed SpringerLink 等数据库收录! |
|