Agrobacterium-mediated transformation of cultivated tomato with construct carrying the nucleoprotein (N) gene from tomato spotted wilt virus (TSWV) |
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| Authors: | O. Fedorowicz G. Bartoszewski P. Stoeva K. Niemirowicz-Szczytt |
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| Affiliation: | (1) Department of Plant Genetics, Breeding and Biotechnology, Warsaw Agricultural University Nowoursynowska 166, 02-787 Warsaw, Poland;(2) Institute of Genetic Engineering, De Monfort University, The Norman Borlaug Centre for Plant Science Research, 2232 Kostinbrod-2, Bulgaria |
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| Abstract: | Significant yield losses in commercial tomato production caused by tomato spotted wilt virus (TSWV) are the reason why we have undertaken studies on resistance to this pathogen. One of the possible sources of resistance can be the incorporation of the nucleoprotein N viral gene by Agrobacterium transformation. The N gene was introduced into three Lycopersicon esculentum forms. Out of the total of 3044 cotyledon explants 14.7% regenerated shoots, but only a few were rooted on medium containing kanamycin. The preliminary analysis indicated that 18 plants are putative transformants. |
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| Keywords: | Lycopersicon esculentum transformants virus resistance |
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