Permanent Mounts Of Chromosomes After 8-Oxyquinoline and Squashing |
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Authors: | A K Sharma Dipti Bhattacharjee |
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Institution: |
a Cytogenetic Laboratory, Department of Botany, Calcutta University, Calcutta 19, India |
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Abstract: | Fresh young root tips or free-hand cross sections thereof were placed in 0.002 M 8-oxyquinoline (aq.) at 10-14oC. for 3 hours. After rinsing in water 1-2 minutes, they were soaked in N HC1 at 55oC. for 25 minutes, rinsed again and squashed under a cover glass on a dry slide. Slide and cover glass were separated by placing in 70% alcohol and allowed to remain therein at least 0.5 hour after separation. Both slide and cover glass were passed through 50% and 30% alcohol to water and stained by the Feulgen procedure (without further hydrolysis) or with crystal violet after mordanting in 1% chromic acid overnight and washing in running water 3-4 hours. Dehydration and mounting in balsam completed the process. The smear on the slide was covered with a clean cover glass and the cover glass, bearing stained material, mounted separately. |
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