仙台病毒黑龙江省地方株的分离与鉴定

目的自本省普通级实验动物中分离并鉴定出仙台病毒地方毒株,为建立仙台病毒血清抗体检测方法奠定基础。方法通过鸡胚尿囊腔传代自普通级小鼠肺脏分离病毒,经血凝实验、血凝阻断实验和结构基因序列测定对分离得到的病毒进行鉴定;大量繁殖病毒并通过蔗糖密度梯度离心纯化,免疫动物制备阳性血清,用标准试剂盒检测阳性血清效价。结果自150份小鼠肺脏分离到2株有血凝性的病毒,经形态学、血清学和结构基因序列测定鉴定为仙台病毒,命名为SV-HLJ。SV-HLJ与标准毒株Fushimi核蛋白基因(N)的核苷酸、氨基酸同源性分别为99·6%和99·0%。结论分离并鉴定出了仙台病毒黑龙江省地方毒株,为检测试剂盒的研制奠定了基础。

Isolation and Identification of Sendai Virus Heilongjiang Strain

Objective To isolate and identify Sendai virus strain in Heilongjiang province, and to be used as a diagnostic antigen in detecting SV serum antibody of laboratory animals. MethodsThe virus was isolated from conventional mice lungs through chicken embryo passages and identified by haemoglutination test, haemoglutination blocking test and constitution genes sequencing. The virus was purified by sugar density gradient centrifugation and was used as an immunogen to laboratory animals. The titers of immune serum were detected by standard kits. ResultsTwo virus strains with haemoglutination characteristics were isolated from 150 portions of mouse lungs, which were identified as Sendai virus through virus morphological characterization, serum detection and constitution gene sequencing. The nucleocapsid (N)gene sequencing result showed that the nucleotide sequence and deduced amino acid sequence of the isolated virus shares 99.6% and 99.0% homology with Fushimi strain, respectively. ConclusionSendai virus HLJ strain has been isolated and identified, which can be used to develop diagnostic kits for SV antibody detection.