链脲佐菌素诱导C57小鼠1型糖尿病模型的研究

目的:通过小剂量多次腹腔注射链脲佐菌素(STZ)诱导建立与人类1型糖尿病相似的C57小鼠糖尿病模型,研究建模剂量和成模率。方法:将32只C57小鼠随机分为正常对照组(A)和实验组(B)。实验组(B)可分为低、中、高剂量组(50 mg/kg、70mg/kg、90 mg/kg)(n=8)。两组都喂普通饲料1周后,B组连续5天腹腔注射不同剂量STZ,测定注射前、注射后1周、2周、3周、4周、5周的空腹血糖和体重,观察小鼠饮食、饮水和排尿情况。STZ注射第3周进行口服糖耐量实验(OGTT)。结果:给药前A、B组体重和血糖无显著差异,给药1周后,B组饮水量和进食量明显增加,体重减轻。C57小鼠用药2周后,中剂量组达到建模标准,成模率75%。各剂量组均出现了糖耐量异常。结论:诱导建立C57小鼠1型糖尿病模型方法是连续5日腹腔注注射STZ,适宜剂量为70 mg/kg。

StudyofType1DiabetesModel InducedbyStreptozotocinintheC57Mouse

Objective:To establish a C57 mouse model of type 1 diabetes by multiple intraperitoneal injection of low concentration STZ, and evaluate model effect and the success rate.Methods:32 C57 mice were randomly divided into normal control group (A) and experimental group (B). The experimental group (B) was divided into low, middle, high dose group (50 mg/kg, 70 mg/kg, 90 mg/kg) (n=8). The two groups were fed with normal diet for 1 week. Group B received different concentration of STZ for 5 consecutive days by intraperitoneal injection. Blood glucose were determined at pre-injection, 1 weeks after injection, 2 weeks, 3 weeks, 4 weeks, 5 weeks and body weight were also detected. At the same time animal''s eating, drinking and voiding were recorded. 3 weeks after STZ injection. Oral glucose tolerance test (OGTT) was carried out at the same time.Results:Body weight and blood glucose group A and group B had no significant difference between group A and group B before drug administration. After treatment for 1 week, mice in group B had water intake and food intake increased, but weight lost. After 2 weeks medication, the C57 mice in middle dose group reached the standard of modeling, and the successful rate was 75%. Each dose group showed an impaired glucose tolerance.Conclusion:The establishment of type 1 diabetes model of C57 mice can be induced by STZ, with consecutive 5 days intraperitoneal injections, and suitable dose of 70 mg/kg.