N-三甲基壳聚糖包裹喜树碱治疗卵巢癌淋巴结转移的研究

目的:采用N-三甲基壳聚糖包裹喜树碱(CPT-TMC)以增加其水溶性,并检测其抑制卵巢癌细胞淋巴结转移的效应。方法:体外实验,PI染色检测CPT-TMC促肿瘤细胞凋亡作用,体内实验,荷瘤裸鼠随机分为4组(5只/组):NS组、TMC组、CPT组及CPT-TMC组,通过尾静脉给药,每周2次,持续3周,Evan's Blue Dye染色,显影腹腔肿大转移的淋巴结。结果:PI染色提示CPT-TMC显著促进肿瘤细胞凋亡(P0.05);Evan's Blue Dye显影显示CPT-TMC显著抑制肿瘤细胞淋巴结转移(P0.05)。结论:CPT-TMC具有明显抑制卵巢癌细胞淋巴结转移效应,它可能成为一种新的有效的卵巢癌治疗药物。

The Impact on Lymph node Metastasis of Ovarian Cancer Based on N-trimethyl Chitosan Chloride-encapsulated Camptothecin

Objective:In the current study, we encapsulated camptothecin with N-trimethyl chitosan (CPT-TMC) to increase its'' solubility and tested it on ovarian cancer model.Methods:For in vitro study, PI staining was performed to evaluate cell apoptosis. For in vivo study, tumor-bearing mice were randomly divided into four groups (5 per group): NS, TMC, CPT and CPT-TMC group, and different group received the corresponding treatment via tail vein twice per week for three weeks. Evan''s Blue Dye was used to indicate lymphatic vessels and swell lymph nodes in abdominopelvic cavity.Results:In vitro, PI staining showed CPT-TMC can significantly induce cell apoptosis (P<0.05). In vivo, CPT-TMC can effectively inhibit metastasis to lymph nodes (P<0.05).Conclusion:The present findings indicate that CPT-TMC may provide an effective approach to inhibit lymph node metastasis of ovarian cancer and it may be a potential novel effective approach to ovarian cancer.