银杏叶提取物对过氧化氢刺激下的血管内皮细胞的影响

目的:探讨银杏叶提取物对血管内皮细胞的保护作用及可能的保护机制。方法:进行血管内皮细胞培养。用过氧化氢(H2O2)处理血管内皮细胞建立细胞凋亡模型。将细胞分为四组:空白对照组、H2O2处理组、单独银杏叶提取物处理组、银杏叶提取物预处理组(提前2h给药后H2O2处理)。进行MTT检测细胞的相对活力、RT-PCR检测目的基因CHOP的表达、Western Blot分析目的蛋白CHOP的表达等。结果:与对照处理组比较,H2O2处理组细胞凋亡率、CHOPmRNA相对表达及CHOP蛋白表达量明显升高。与H2O2处理组比较,银杏叶提取物预处理组细胞凋亡指数、CHOPmRNA相对表达及CHOP蛋白表达量明显降低(P0.05)。结论:作为一种清除自由基、抗氧化、抗衰老药物,银杏叶提取物可能通过调节CHOP蛋白选择性地抑制过度的内质网应激来保护血管内皮细胞。

Effects of Ginkgo Biloba Extract on Hydrogen Peroxide-stimulated Vascular Endothelial Cells

Objective:To investigate the protective effects of the Ginkgo biloba extract on vascular endothelial cells and its possible mechanisms.Methods:The endothelial cells of blood vessels were cultured. The endothelial cells apoptosis model was established by treatment with hydrogen peroxide (H2O2). Then divide the cells into four groups: blank control group, H2O2-treated group, single ginkgo biloba extract-treated group, ginkgo biloba extract pretreatment group (giving H2O2 at 2 h after administration of ginkgo biloba extract). The relative vitality were determined by MTT test cells. The expressions of gene CHOP were detected by RT - PCR, and the CHOP target protein expressions were detected by western blot analysis.Results:Compared with the control, the cells apoptosis rate, CHOPmRNA relative expression and CHOP protein expression was significantly increased in the H2O2-treated group. Compared with H2O2-treated group, the cells apoptosis rate, CHOPmRNA relative expression and CHOP protein expression was significantly reduced in the ginkgo biloba extract pretreatment group (P<0.05).Conclusion:As an antioxidant, free-radical scavenging and anti-aging medicine, Ginkgo biloba extract may protect endothelial cells by regulating CHOP protein and selectively inhibiting excessive endoplasmic reticulumstress.