Recombination hot spots within the I region of the mouse H-2 complex map to the E
β
and E
α
genes |
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Authors: | William P Lafuse Chella S David |
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Institution: | (1) Department of Immunology, Mayo Clinic and Medical School, Rochester, Minnesota, USA;(2) Present address: Department of Medical Microbiology and Inununology, Ohio State University, 333 West 10th Avenue, 43210 Columbus, OH, USA |
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Abstract: | Recombinant mouse strains with crossovers in the I region of the H-2 major histocompatibility complex were examined by restriction fragment analysis for the presence of polymorphic restriction sites within the E
and E
genes. Nine recombinant mouse strains were shown to have crossed over within a 5 kb DNA segment that contains the large intron between the second and third exons of the E
gene. These results are in accord with previous studies mapping a recombination hot spot within this gene. Seven recombinant mouse strains between the p and k haplotypes were shown to have crossed over in a 6 kb segment within the E
gene. These results show the existence of a recombination hot spot within the E
gene. Comparison of the H-2 haplotypes involved in these two recombination hot spots suggests that a specific DNA sequence in b, s, f, and q haplotypes may act to promote recombination in the E
gene and a specific DNA sequence in the p haplotype may act to promote recombination in the E
gene. |
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Keywords: | |
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