Plantlet formation from isolated protoplasts ofSolanum melongena L. |
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Authors: | P K Saxena R Gill A Rashid S C Maheshwari |
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Institution: | (1) Department of Botany, University of Delhi, 110007 Dehli, India |
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Abstract: | Summary Mesophyll protoplasts were isolated from axenic shoot cultures ofSolanum melongena by the one-step enzymatic method. Of the different media employed for the culture of protoplasts, a medium modified fromKao andMichayluk (1975) supported sustained mitotic cycles most effectively. Organogenesis from protoplast-derived callus was achieved on transfer toMurashige andSkoog'S (1962) medium supplemented with an appropriate auxin and a cytokinin. |
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Keywords: | Solanum melongena Isolated protoplasts Regeneration |
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