Porphyromonas gingivalis with either Tannerella forsythia or Treponema denticola induces synergistic IL-6 production by murine macrophage-like J774.1 cells |
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| Institution: | 1. Medical School of Dicle University, Department of Cardiovascular Surgery, Diyarbakir, Turkey;2. Istanbul Bilim University, Sisli Florence Nightingale Hospital, Department of Cardiovascular Surgery, Istanbul, Turkey;3. Medical School of Dicle University, Department of Biochemistry, Diyarbakir, Turkey;1. Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Instituto Agroalimentario de Aragón (IA2), Universidad de Zaragoza-CITA, Zaragoza, Spain;2. Protein Chemistry Laboratory, Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark;1. Department of Prosthodontics and Oral Rehabilitation, Graduate School of Dentistry, Kanagawa Dental University, Inaoka 82, Yokosuka, Kanagawa 238-8580, Japan;2. Department of Materials Science, Graduate School of Pure and Applied Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki 305-8573, Japan;3. Department of Microbiology, Graduate School of Dentistry, Kanagawa Dental University, Inaoka 82, Yokosuka, Kanagawa 238-8580, Japan;4. Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University, Inaoka 82, Yokosuka, Kanagawa 238-8580, Japan;5. Department of Radiopraxis Science, Graduate School of Dentistry, Kanagawa Dental University, Inaoka 82, Yokosuka, Kanagawa 238-8580, Japan;6. Yokosuka-shonan Disaster Health Emergency Research Center and ESR Laboratories, Graduate School of Dentistry, Kanagawa Dental University, Inaoka 82, Yokosuka, Kanagawa 238-8580, Japan;7. Master''s School of Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki 305-8573, Japan;8. Satellite Laboratory, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki 305-8573, Japan;1. Department of Microbiology, Penn Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA;2. Department of Oral Immunology and Infectious Diseases, School of Dentistry, University of Louisville, Louisville, KY, USA |
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| Abstract: | BackgroundChronic periodontitis is caused by mixed bacterial infection. Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola are frequently detected in deep periodontal pockets. We demonstrate that these bacteria induce proinflammatory cytokine production by the mouse macrophage-like cell line J774.1.Materials and methodsJ774.1 cells were incubated with and without bacteria for 24 h in 96-well flat-bottomed plates. The culture supernatants were analyzed by enzyme-linked immunosorbent assay for secreted mouse interleukin (IL)-6, monocyte chemoattractant protein-1, IL-23, IL-1β and tumor necrosis factor-α. The cytokine concentrations were determined using a standard curve prepared for each assay.ResultsMixed infection with P. gingivalis and either T. forsythia or T. denticola at 105 CFU/ml acted synergistically to increase IL-6 production, but not monocyte chemoattractant protein-1, IL-23, IL-1β or tumor necrosis factor-α production. Gingipain inhibitors KYT-1 and KYT-36 inhibited IL-6 production by J774.1 cells incubated with 105 CFU/ml of mixed bacteria.ConclusionThese results suggest that P. gingivalis with either T. forsythia or T. denticola directly induces synergistic IL-6 protein production and that gingipains play a role in this synergistic effect. |
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