Leptospira interrogans requires heme oxygenase for disease pathogenesis |
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| Affiliation: | 1. Laboratório Integrado de Morfologia, Núcleo em Ecologia e Desenvolvimento Sócio Ambiental de Macaé – NUPEM, Universidade Federal do Rio de Janeiro, Campus UFRJ - Macaé, Macaé, RJ, Brazil;2. Laboratório de Neurobiologia Comparativa e do Desenvolvimento, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, UFRJ, Rio de Janeiro, RJ, Brazil;3. Instituto de Pesquisas, Jardim Botânico do Rio de Janeiro, Rio de Janeiro, RJ, Brazil;4. Pós-Graduação em Produtos Bioativos e Biociências, Universidade Federal do Rio de Janeiro, Campus UFRJ - Macaé, Macaé, RJ, Brazil |
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| Abstract: | We recently characterised the Leptospira interrogans heme oxygenase (hemO) gene and showed that HemO was required for growth with hemoglobin as the sole iron source. Here we investigated the role of HemO in pathogenesis. Hamsters inoculated with the hemO mutant showed 83% survival, compared with 33% for a control mutant (intergenic transposon insertion). Lung pathology was consistent with survival data, showing that HemO contributes significantly to pathogenesis and heme is a major in vivo iron source for L. interrogans. This is only the second defined, attenuated mutant in pathogenic Leptospira and the first to define function of the mutated gene. |
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