Isolation and characterization of polymorphic microsatellite DNA makers for Euphrasia nankotaizanensis (Orobanchaceae) and cross amplification in another Euphrasia L. |
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Authors: | Kuo-Hsiung Wang Ming-Jou Wu Tzen-Yuh Chiang Chang-Hung Chou |
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Institution: | (1) Department of Life Sciences, National Cheng Kung University, Tainan, 701, Taiwan, ROC;(2) Graduate Institute of Biological Resources and Technology, National Dong Hwa University, Hualien, 970, Taiwan, ROC;(3) Research Center for Biodiversity, Graduate Institute of Ecology and Evolutionary Biology, China Medical University, Taichung, 404, Taiwan, ROC |
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Abstract: | Euphrasia nankotaizanensis is an endangered flowering plant distributed restrictedly on rocky slope of high mountain peaks in central and northern Taiwan.
In order to undertake a conservation program, especially given impacts of the global warming, it is essential to evaluate
its genetic diversity and population structure. We described nine novel microsatellite primer pairs in E. nankotaizanensis and also examined its relative E. transmorrisonensis. The number of alleles per locus ranged from 7 to 29. Expected (H
E) and observed (H
O) heterozygosities ranged from 0.83 to 0.98 and 0.00 to 0.95, respectively. Eight of the nine microsatellite loci displayed
significant departures from Hardy–Weinberg expectations, likely due to the loss of habitats and the small population size.
Significant linkage disequilibrium (LD) was detected in most loci. Cross-species amplification of microsatellites took place
at five loci. These primers may provide a tool for understanding the demography and population structure of Euphrasia species in Taiwan.
Kuo-Hsiung Wang, Ming-Jou Wu, and Tzen-Yuh Chiang contributed equally to this work. |
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Keywords: | Euphrasia Microsatellite PIMA RAPD-PCR enrichment Taiwan |
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