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Creation of DNA overhangs by using modified DNA overhang cloning method
Authors:Wei Li  Feng Gao
Institution:(1) Laboratory of Surgery, The First Affiliated Hospital of Guangxi Medical University, No. 22, Shuangyong Road, Nanning, Guangxi, 530021, People’s Republic of China;(2) Medical Science Research Center, Guangxi Medical University, No. 22, Shuangyong Road, Nanning, Guangxi, 530021, People’s Republic of China;(3) Department of Anal and Colorectal Surgery, Guangxi Medical University, No. 22, Shuangyong Road, Nanning, Guangxi, 530021, People’s Republic of China
Abstract:
Keywords:DNA overhang  Seamless gene engineering  Gene synthesis  RNA–  DNA primer  PCR  DNA ligation
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