脂联素基因剔除小鼠糖代谢和骨代谢的初步研究

目的:研究前期建立的脂联素基因剔除小鼠模型在基础状态的葡萄糖代谢及骨代谢状态。方法:采用长期追踪野生型和该基因剔除纯合子小鼠的体重和空腹血糖水平,并进行葡萄糖耐量(GTT)和胰岛素耐量(ITT)试验以评价该小鼠的葡萄糖代谢能力。对骨代谢的初步研究采用检测小鼠骨密度(BMD)的方式。结果:发现该基因剔除小鼠在6w时,出现空腹血糖比对照组显著低下的现象,而随着鼠龄的增长,血糖水平又趋于正常。GTT和ITT试验证明该基因剔除小鼠无糖尿病或胰岛素抵抗表型。BMD检测亦显示该小鼠在基础状态下无骨密度低下的现象。结论:脂联素基因剔除小鼠在基础状态下并无显著的糖代谢和骨代谢异常。

Preliminary Study on Glycometabolism and Bone Metabolism in Adiponectin Gene Knockout Mice

Objective:To study the glycometabolism and bone metabolismstatus in adiponectin(APN) gene knockoutmice.Meth-ods:We detected the weight and glucose level in APN gene knockout mice and evaluated the ability of glycometabolism by GTT and ITT.We also detected BMD in APN gene knockout mice to evaluate the relationship between adiponectin and bone metabolism.Results:There's a significant difference between 6-week-old APN gene knockout mice and control mice in glucose level.GTT, ITT and BMD showed no difference between wild type and mutant mice.Conclusion:The results shows that no significant alteration on glycometabolism or bone metabolism was found in APN gene knockout mice.