Expression of stilbene synthase gene in transgenic tomato using salicylic acid-inducible Cre/loxP recombination system with self-excision of selectable marker |
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Authors: | Ma B G Duan X Y Niu J X Ma C Hao Q N Zhang L X Zhang H P |
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Institution: | (1) Key Oasis Eco-agriculture Laboratory of Xinjiang Production and Construction Group, Agricultural College, Shihezi University, Shihezi, 832000, People’s Republic of China;(2) Department of Horticulture, Agricultural College, Shihezi University, Shihezi, 832000, People’s Republic of China |
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Abstract: | A plant transformation vector, pCLKSCLA25 (EU327498), was developed to contain eight cloning sites and the inducible self-excision
system which provided an effective approach to eliminate the selectable marker gene(s) from transgenic plants. Upon induction
by salicylic acid, the cre gene produced a recombinase that eliminated sequences encoding the selectable marker neomycin phosphotransferase and cre itself. The excision efficiency was 41% in transgenic tomato regenarants. The stilbene synthase gene (vst1) from Vitis vinifera L. was cloned into pCLKSCLA25. The expression of vst1 gene contributed to the accumulation of trans-reveratrol from 3.4 to 8.7 μg/g fresh wt in different marker-free transgenic tomato lines.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Cre/loxP recombination system Grape Resveratrol Self-excision Stilbene synttase gene Transgenic tomato Vitis vinifera |
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