An assessment of soil bacterial community structure and physicochemistry in two microtopographic locations of a palustrine forested wetland |
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Authors: | Changwoo Ahn Patrick M Gillevet Masoumeh Sikaroodi Kristin L Wolf |
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Institution: | (1) Department of Environmental Science and Policy, George Mason University, 4400 University Dr., MS 5F2, Fairfax, VA 22030, USA |
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Abstract: | We studied redoximorphic features, field indicators and bacterial communities of soils in hummocks and hollows of a palustrine
forested wetland in Virginia. We hypothesized that presence of hydric soils, soil physicochemistry and soil bacterial community
structure would differ between hummocks and hollows. We fingerprinted soils collected from different microtopographic locations
using Length Heterogeneity Polymerase Chain Reaction (LH-PCR) to study their bacterial community structures. Two hummocks
had silty/sandy loam soils with mean chroma values of > 4, showing no indication of ‘hydric soils’ (i.e., wetland soils).
Two hollows, however, had clay loam soils with mean chroma values of 2 with gleying and redox concentrations observed, indicative
of seasonally inundated wetlands. The soils of hollows also had higher organic matter content and soil moisture compared to
the soils of hummocks (P < 0.05). Multidimensional scaling (MDS) and Analysis of similarity (ANOSIM) of the fingerprints revealed differences in soil
microbial community structures between hummocks and hollows (Global R = 0.30, P < 0.01). The diversity measures of the fingerprints (Shannon’s H′) were also different by microtopography with higher diversity
in hollows relative to hummocks (P < 0.05). LH-PCR proves to be a useful tool in examining bacterial community composition of wetland soils in this study. However,
cloning and sequencing of specific community LH-PCR profiles of interest is necessary to fully characterize the community
down to genus/species level. With species identities we should be able to not only better explain differences observed in
the community profiles, but study their relations to hydrologic and/or physicochemical conditions of wetlands. |
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Keywords: | Soil bacterial community structure LH-PCR Forested wetlands Microtopography Redoximorphic features Wetland soils |
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