| 耕作和施肥对不同深度黑土中细菌群落结构的影响 |
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| 引用本文： | 高崇洋,赵阳国,王爱杰,任南琪,白洁,田伟君,张健,李海艳.耕作和施肥对不同深度黑土中细菌群落结构的影响[J].微生物学报,2010,50(1):67-75. |
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| 作者姓名： | 高崇洋 赵阳国 王爱杰 任南琪 白洁 田伟君 张健 李海艳 |
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| 作者单位： | 1. 哈尔滨工业大学市政环境工程学院,哈尔滨,150090
2. 中国海洋大学环境科学与工程学院,青岛,266100 |
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| 基金项目： | 水体污染控制与治理科技重大专项(2008ZX07208-009);国家自然科学基金(40801193) |
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| 摘 要： | 【目的】为探讨耕作和施加有机肥、化肥对黑土表层(0-30cm)、中层(100-130cm)及深层(200-230cm)细菌群落结构的影响,【方法】应用DGGE技术对相应土层中细菌群落结构进行了解析。【结果】结果表明,与对照相比,耕作和施加有机肥、化肥对表层黑土细菌群落结构影响较大,二者差异度为4%;而对中层和深层细菌群落结构影响较小,二者差异度为2%。对于细菌群落结构的垂向变化,中层和深层中细菌群落结构的相似性远远高于同表层的相似性。【结论】可见,耕作和施加有机肥、化肥仅对黑土表层土壤(0-30cm)具有一定的影响,而对100cm以下土壤细菌群落影响较小,细菌群落随土壤深度不同的垂向变化要远高于土壤管理措施造成的影响。
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| 关 键 词： | 关键词：黑土，群落结构，变性梯度凝胶电泳 |
| 收稿时间： | 7/5/2009 12:00:00 AM |
| 修稿时间： | 2009/9/18 0:00:00 |
Effect of tillage and fertilization on bacterial community structure in different depth of black soil |
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| Chongyang Gao,Yangguo Zhao,Aijie Wang,Nanqi Ren,Jie Bai,Weijun Tian,Jian Zhang and Haiyan Li.Effect of tillage and fertilization on bacterial community structure in different depth of black soil[J].Acta Microbiologica Sinica,2010,50(1):67-75. |
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| Authors: | Chongyang Gao Yangguo Zhao Aijie Wang Nanqi Ren Jie Bai Weijun Tian Jian Zhang and Haiyan Li |
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| Institution: | School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China;College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China;School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China;College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China |
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| Abstract: | Objective] To analyze the impact of tillage and fertilization on bacterial community structure under different depth in black soil.Methods] We used denaturing gradient gel electrophoresis (DGGE) profile analysis of bacterial community based on the 16S rRNA gene combining soil physico-chemical characteristics. Results] DGGE profiles showed that tillage and fertilization influenced more on bacterial community structrue in the surface soil than the subsurface and deep soil,and the divergence distance betwee... |
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| Keywords: | Keywords: black soil community structure denaturing gradient gel electrophoresis (DGGE) |
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