| 缺血引起沙土鼠纹状体DARPP—32磷酸化状态的变化 |
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| 作者姓名： | Sun YF Tang FM Wang R Zhang GY Jin GZ |
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| 作者单位： | 1. 徐州医学院生物化学与分子生物学研究中心,
2. 中国科学院上海药物研究所, |
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| 基金项目： | This work was supported
by the Provincial Natural Science Foundation of Jiangsu, (BK97153) and the Natural Science
Foundation of Education Committee of Jiangsu Province (98KJB310005). |
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| 摘 要： | 采用蒙古沙土双侧颈总动脉阻断前脑缺血模型,以放射自显影（反向磷酸化,back-phosphorylation)及免疫印迹（Western blotting)法体外测定缺血时纹状体DARPP－32磷酸化水平和蛋白含量的变化,结果表明,短暂性缺血纹状体DARPP－32的免疫学活性和蛋白含量地明显改变。在缺血10min内,随缺血时间的延长,体外DARPP－32的^32P]的掺入量在缺血5min时升高,在缺血2,7,10min时均降低,而反向磷酸化的测定结果表明体内DARPP－32磷酸化水平增高,说明缺血可诱导DARPP－32磷酸化水平变化。
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| 关 键 词： | 脑缺血 蒙古沙土鼠 DARPP－32 反向磷酸化 纹状体 |
| 修稿时间： | 2000年7月6日 |
Changes in the state of phosphorylation of DARPP-32 of gerbil striatum during ischemia |
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| Sun YF,Tang FM,Wang R,Zhang GY,Jin GZ.Changes in the state of phosphorylation of DARPP-32 of gerbil striatum during ischemia[J].Acta Physiologica Sinica,2001,53(2):108-110. |
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| Authors: | Sun Y F Tang F M Wang R Zhang G Y Jin G Z |
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| Institution: | Research Center of Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou 221002. |
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| Abstract: | Using the mongolian gerbil model of transient forebrain ischemia by bilateral carotid arteries occlusion, the levels of phosphorylation and the amount of DARPP 32 in striatum were measured by back phosphorylation and Western blotting assay in vitro . Transient forebrain ischemia had no effect on the immunoreaction of DARPP 32 in striatum, but the state of phosphorylation of DARPP 32 showed a significant change. The 32 P] phosphate incorporation in DARPP 32 decreased after 2, 7 or 10 min of ischemia, but increased after a 5 min ischemia. In contrast, the result of back phosphorylation in vivo was opposite to that obtained in vitro .WT5HZ] |
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| Keywords: | cerebral ischemia mongolian gerbil DARPP-32 back phosphorylation striatum Western blotting |
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