Nickel resistance in fission yeast associated with the magnesium transport system |
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| Authors: | Aysegul Topal Sarikaya Gokhan Akman Guler Temizkan |
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| Institution: | (1) Faculty of Science, Department of Moleuclar Biology and Genetics, Istanbul University, 34118 Vezneciler Istanbul, Turkey;(2) Instanbul University Research and Application Center for Biotechnology and Genetic Engineering, 34118 Vezneciler, Istanbul, Turkey;(3) Hasan Ali Yucel Education Faculty, Department of Primary Education, Division of Science Teacher Training, Istanbul University, 34118 Vezneciler, Turkey |
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| Abstract: | We isolated and characterized a nickel (Ni2+)-resistant mutant (GA1) of Schizosaccharomyces pombe. This mutant strain displayed resistance to both Ni2+ and Zn2+, but not to Cd2+, Co2+, and Cu2+. The growth rate of GA1 increased proportionally with increasing Mg2+ concentrations until 50 mM Mg2+. The GA1 mutation phenotype suggests a defect in Mg2+ uptake. Sequence analysis of the GA1 open reading frame (ORF) O13779, which is homologous to the prokaryotic and eukaryotic CorA Mg2+ transport systems, revealed a point mutation at codon 153 (ccc to acc) resulting in a Pro 153Thr substitution in the N-terminus
of the CorA domain. Our results provide novel genetic information about Ni2+ resistance in fission yeast. Specifically, that reducing Mg2+ influx through the CorA Mg2+ transport membrane protein confers Ni2+ resistance in S. pombe. We also report that Ni2+ ion detoxification of the fission yeast is related to histidine metabolism and pH. |
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| Keywords: | Schizosaccharomyces pombe nickel resistance Mg2+ transport system metal transport |
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