An enzymatic fluorometric assay for pyridoxal with high specificity and sensitivity |
| |
| Authors: | Trongpanich Yanee Mito Mitsunori Yagi Toshiharu |
| |
| Institution: | Department of Bioresources Science, Faculty of Agriculture, Kochi University, Japan. |
| |
| Abstract: | An enzymatic fluorometric assay for pyridoxal with pyridoxal dehydrogenase was developed. The detection limit was about 10 pmol: the calibration curve of pyridoxal showed high linearity (r=0.993). The values obtained by this method correlated well with those by the HPLC method. The enzyme had a high specificity for pyridoxal, and thus animal samples could be directly analyzed without separation of pyridoxal 5'-phosphate by column chromatography. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
