Soluble serum CD81 is elevated in patients with chronic hepatitis C and correlates with alanine aminotransferase serum activity |
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Authors: | Welker Martin-Walter Reichert David Susser Simone Sarrazin Christoph Martinez Yolanda Herrmann Eva Zeuzem Stefan Piiper Albrecht Kronenberger Bernd |
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Institution: | 1. Medizinische Klinik 1, Klinikum der Johann Wolfgang Goethe-Universität, Theodor-Stern-Kai 7, Frankfurt am Main, Germany.; 2. Fachbereich Medizin, Institut für Biostatistik und mathematische Modellierung, Johann Wolfang Goethe-Universität, Frankfurt am Main, Germany.;Karolinska Institutet, Sweden |
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Abstract: | AimCellular CD81 is a well characterized hepatitis C virus (HCV) entry factor, while the relevance of soluble exosomal CD81 in HCV pathogenesis is poorly defined. We performed a case-control study to investigate whether soluble CD81 in the exosomal serum fraction is associated with HCV replication and inflammatory activity.Patients and MethodsFour cohorts were investigated, patients with chronic hepatitis C (n?=?37), patients with chronic HCV infection and persistently normal ALT levels (n?=?24), patients with long term sustained virologic response (SVR, n?=?7), and healthy volunteers (n?=?23). Concentration of soluble CD81 was assessed semi-quantitatively after differential centrifugation ranging from 200 g to 100,000 g in the fifth centrifugation fraction by immunoblotting and densitometry.ResultsSoluble CD81 was increased in patients with chronic hepatitis C compared to healthy subjects (p?=?0.03) and cured patients (p?=?0.017). Patients with chronic HCV infection and persistently normal ALT levels and patients with long term SVR had similar soluble CD81 levels as healthy controls (p>0.2). Overall, soluble CD81 levels were associated with ALT levels (r?=?0.334, p?=?0.016) and severe liver fibrosis (p?=?0.027).ConclusionCD81 is increased in the exosomal serum fraction in patients with chronic hepatitis C and appears to be associated with inflammatory activity and severity of fibrosis. |
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