Rapid identification and authentication of closely related animal cell culture by polymerase chain reaction |
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Authors: | Merry Liu Hsi Liu Xiaoling Tang Abbas Vafai |
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Institution: | National Center for Preparedness, Detection and Control of Infectious Diseases, Centers for Disease Control and Prevention, MS-D34, 1600 Clifton Road, Atlanta, GA 30333, USA. |
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Abstract: | Animal cell lines are important resources for research and diagnostic applications. Cross-contamination and misidentification
of cell lines, however, can cause major problems for research (for example, false results that come from contamination cells
may mislead the science). Hence, it is imperative to routinely monitor cell lines for identity and authenticity. Here, we
extend our previous work on identification and authentication of animal cell culture by polymerase chain reaction (PCR) amplification
and DNA sequencing. A PCR-based method for rapid identification and authentication of closely related cell lines was described.
In this method, two new primers were designed based on high homology in the aldolase gene family. Used together with our previous
primers, the combinations of primers were able to differentiate closely related species, including human from monkey and mouse
from rat. This PCR assay provides a rapid, simple, sensitive, and cost-effective method for authentication of closely related
cell lines.
The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the funding
agency. |
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Keywords: | PCR Cell lines Authentication |
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