| 一株分枝杆菌降解胆固醇制备AD(D)的转化条件 |
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| 引用本文： | 王敏,温竹,王丽娟,申雁冰,王洪臣.一株分枝杆菌降解胆固醇制备AD(D)的转化条件[J].生物加工过程,2008,6(2):22-27. |
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| 作者姓名： | 王敏 温竹 王丽娟 申雁冰 王洪臣 |
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| 作者单位： | 天津市工业微生物重点实验室,天津科技大学,生物工程学院,天津,300457 |
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| 基金项目： | 国家自然科学基金
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天津市应用基础研究重点项目 |
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| 摘 要： | 通过分枝杆菌(Mycobacteriumsp.)M3限制性降解胆固醇侧链获得了产物雄甾-4-烯-3,17-二酮(AD)和雄甾-1,4-二烯-3,17-二酮(ADD)。优化了胆固醇的投料时间、投料方式、培养基初始pH和葡萄糖浓度等工艺参数。将羟丙基-β-环糊精(HP-β-CD)应用于转化反应中,确定了HP-β-CD的最佳添加时间和添加量,使AD(D)生成率由初始对照的30%提高到60%,转化至72 h时AD(D)生成率达48%,是同期对照的4.0倍,生成率与生成速率均得到显著提高。在添加HP-β-CD的最佳转化条件下,AD(D)生成率达到70%,是初始对照的2.3倍。
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| 关 键 词： | 雄甾-2-2烯-3 17-二酮 胆固醇 生物转化 分枝杆菌 |
| 文章编号： | 1672-3678(2008)02-0022-06 |
| 修稿时间： | 2007年11月23 |
The bioconversion conditionsof cholesterol cleavage by Mycobacterium sp. M3 |
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| WANG Min,WEN Zhu,WANG Li-juan,SHEN Yan-bing,WANG Hong-chen.The bioconversion conditionsof cholesterol cleavage by Mycobacterium sp. M3[J].Chinese Journal of Bioprocess Engineering,2008,6(2):22-27. |
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| Authors: | WANG Min WEN Zhu WANG Li-juan SHEN Yan-bing WANG Hong-chen |
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| Institution: | WANG Min; WEN Zhu; WANG Li-juan; SHEN Yan-bing; WANG Hong-chen (Laboatory of Microbial Pharmacy; College of Biotechnology; Tianjin University of Science & Technology; Tianjin Key Laboratory of Industrial Microbiology; Tianjin 200457; China); |
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| Abstract: | Cholesterol can be cleavaged by Mycobacterium sp.M3 to androst-4-ene-3,17-dione AD(D) and androsta-,4-diene-3,17-dione(ADD).Fermentation parameters such as substrate addition time,initial pH,and concentration of glucose in the fermentation culture were optimized.The conversion rate of AD(D) had been raised to 60% from the original 30% in the presence of HP-β-CD,and it was 4 times to the contrast when the conversion time was 72 h,the conversion rate and speed of AD(D) had been obviously raised.In the optimiz... |
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| Keywords: | androstα-1 4-diene-3 17-dione cholesterol bioconversion Mycobacterium sp M3 |
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