肥大细胞在大鼠输卵管急性沙眼衣原体感染中的作用

目的　为研究肥大细胞在大鼠输卵管急性沙眼衣原体 (Chlamydialtrachomatis,CT)感染中的作用。方法　选择成年雌性SD大鼠 6 0只 ,通过手术从一侧卵巢囊接种沙眼衣原体D型株 ,对照组接种 2 -SPA缓冲液。分别于感染后第 1/ 2d、第 7d、第 14d将大鼠处死 ,取手术侧的输卵管常规固定、脱水、包埋。结果　S -P法显示 :输卵管局部的CD4 + T细胞和血管内皮细胞粘附分子 (VCAM - 1)的表达均较对照组明显增强 (P <0 0 1)。改良的甲苯胺蓝染色法显示 :感染组肥大细胞较对照组数量有显著性增高 (P <0 0 5 ) ,并且其变化趋势与CD4 + T细胞和VCAM - 1表达的变化趋势一致。结论　可以推测 ,沙眼衣原体感染引起急性输卵管炎时 ,肥大细胞通过促进炎症局部小静脉内皮细胞上VCAM -1的表达 ,诱导CD4 + T细胞的浸润 ;然后分泌IL - 4等细胞因子促进CD4 + T细胞向TH2 细胞方向转化 ,不利于机体清除沙眼衣原体 ,从而使发生局部输卵管病理损伤的可能性增加。

INFLUENCE OF MAST CELLS IN THE OVIDUCT OF FEMALE RATS AFTER CHLAMYDIAL INFECTION

Objective To study the relationship between mast cells and chlamydial infections in the oviduct of the rat.Method Sixty adult female S.D. rats were inoculated chlamydia serum D through the ovary blast, and were killed 1/2 day, 7 days and 14 days after infection. Mast cells were detected by an improved toluidine blue staining method and CD 4 + T cells, and vascular cell adhesion molecules (VCAM-1) by immunohistochemistry. Result In the infected groups, the number of the positive CD 4 +T cells and VCAM-1 increased significantly P<0.01 when compared to the control groups. And the changes of mast cells were related with CD 4 +T cells and VCAM-1. Conclusion Mast cells may promote the expression of VCAM-1, which may indicate the infiltration of T cells in the local inflammation. Furthermore, mast cells may promote the transformation of T cells into Th2 cells by secreting IL-4. We know that it is Th1 but not Th2 cells that play an important role in controlling the inflammation of chlamydial trachomatis in the oviduct. As a result of the disequilibrium of Th1 and Th2, the infection of CT in the oviduct will be prolonged and servely damage the tissues.