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In vivo detection of intrinsic reactive oxygen species using acyl-protected hydroxylamine in puromycin nephrosis |
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| Authors: | Atsushi Ueda Sohji Nagase Mariko Inoue Takaaki Oteki Masaaki Aoyama |
| Affiliation: | 1. Department of Internal Medicine, Namegata District General Hospital, 98-8 Inoue-Fujii, Namegata, Ibaraki, 311-3516, Japan;2. Pathophysiology of Renal Diseases, Medical Sciences for Control of Pathological Processes, Graduate School of Comprehensive Human Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan;3. Center for Clinical Medicine and Research, International University of Health and Welfare, 2600-1 Kitakanamaru, Ohtawara, Tochigi, 324-8501, Japan;4. Yamagata Promotional Organization for Industrial Technology, 2-2-1 Matsuei, Yamagata, 990-2473, Japan |
| Abstract: | Intrinsic reactive oxygen species (ROS) in a rat model of human minimal change nephropathy were detected directly using an in vivo electron paramagnetic resonance (EPR) method with 1-acetoxy-3-carbamoyl-2,2,5,5-tetramethylpyrrolidine (ACP) in real time. The nephrosis was induced by the intravenous administration of 75 mg/kg of puromycin aminonucleoside (PAN). It was found that ROS in the kidney were increased 1 h after the administration of PAN. This increased oxidative stress declined at 24 h and returned to a normal level 3 days after PAN administration. This is the first non-invasive in vivo detection and quantification of specific ROS in an experimental nephrosis model. |
| Keywords: | Reactive oxygen species acyl-protected hydroxylamine puromycin aminonucleoside nephrosis electron paramagnetic resonance |