Resveratrol inhibition of lipid peroxidation |
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Authors: | Bruna Tadolini Claudia Juliano Luisella Piu Flavia Franconi Luciana Cabrini |
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Institution: | 1. Dipartimento di Scienze Biomediche, Sezione di Biochimica, Italytadolini@ssmain.uniss.it;3. Dipartimento di Scienze del Farmaco, Università di Sassari, Italy;4. Istituto Nazionale Biostrutture e Biosistemi, Osilo, Sassari, Italy;5. Dipartimento di Biochimica, Università di Bologna, Italy;6. Dipartimento di Scienze Biomediche, Sezione di Biochimica, Italy |
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Abstract: | To define the molecular mechanism(s) of resveratrol inhibition of lipid peroxidation we have utilized model systems that allow us to study the different reactions involved in this complex process. Resveratrol proved (a) to inhibit more efficiently than either Trolox or ascorbate the Fe2+ catalyzed lipid hydroperoxide-dependent peroxidation of sonicated phosphatidylcholine liposomes; (b) to be less effective than Trolox in inhibiting lipid peroxidation initiated by the water soluble AAPH peroxyl radicals; (c) when exogenously added to liposomes, to be more potent than α-tocopherol and Trolox, in the inhibition of peroxidation initiated by the lipid soluble AMVN peroxyl radicals; (d) when incorporated within liposomes, to be a less potent chain-breaking antioxidant than α-tocopherol; (e) to be a weaker antiradical than α-tocopherol in the reduction of the stable radical DPPH·. Resveratrol reduced Fe3+ but its reduction rate was much slower than that observed in the presence of either ascorbate or Trolox. However, at the concentration inhibiting iron catalyzed lipid peroxidation, resveratrol did not significantly reduce Fe3+, contrary to ascorbate. In their complex, our data indicate that resveratrol inhibits lipid peroxidation mainly by scavenging lipid peroxyl radicals within the membrane, like α-tocopherol. Although it is less effective, its capacity of spontaneously entering the lipid environment confers on it great antioxidant potential. |
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Keywords: | Resveratrol lipid peroxidation iron AAPH AMVN phosphatidylcholine liposomes |
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