The steady-state levels of oxidative DNA damage and of lipid peroxidation (F2-isoprostanes) are not correlated in healthy human subjects |
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| Authors: | Timothy England Emily Beatty Almas Rehman Jaffar Nourooz-Zadeh Paulo Pereira James O'Reilly |
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| Affiliation: | 1. International Antioxidant Research Centre, King's College, Guys Campus, London Bridge, London, SE1 9RT, UK;2. Nutrition, Food and Health Research Centre, King's College, Franklin-Wilkins Building, 150 Stamford Street, London, SE1 8WA, UK;3. Centre for Clinical Pharmacology and Therapeutic Toxicology, Department of Medicine, University College, London, WC1E 6JJ, UK;4. Department of Ophthalmology and Visual Science, Biomedical Institute for Research in Light and Image, Coimbra, Portugal;5. Department of Biochemistry, National University of Singapore, 10 Kent Ridge Crescent, Singapore, 119260 |
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| Abstract: | ![]()
Oxidative damage to DNA in human tissues can be determined by measuring multiple products of oxidative damage to the purine and pyrimidine bases using gas chromatography-mass spectrometry (GC-MS). Oxidative damage to lipids (lipid peroxidation) can be quantitated by the mass spectrometry-based determination of F2-isoprostanes, specific end-products of the peroxidation of arachidonic acid residues in lipids. For both DNA base damage products and 8-epi prostaglandin F2α (PGF2α), there is a wide variation in levels between different healthy human subjects. We measured multiple products of oxidative damage to DNA bases in white cells, and 8-epi PGF2α in plasma, from blood samples obtained from healthy human subjects in the UK and in Portugal. No correlation of 8-epi PGF2α levels with levels of any modified DNA base (including 8-hydroxyguanine) was observed. We conclude that no single parameter can be measured as an index of “oxidative stress” or “oxidative damage” in vivo. |
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| Keywords: | Oxidative DNA damage lipid peroxidation isoprostanes F2-isoprostanes 8-hydroxyguanine antioxidant |
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