Hydrogen-rich saline protects against intestinal ischemia/reperfusion injury in rats |
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| Authors: | Xingfeng Zheng Xingfeng Zheng Yanfei Mao Jianmei Cai Yonghua Li Wenwu Liu |
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| Affiliation: | 1. Chinese PLA Institute of Burn Surgery &2. Department of Burn Surgery, Changhai Hospital, Second Military Medical University, Shanghai, PR China;3. Department of SICU, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, PR China;4. Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, PR China;5. Department of Anaesthesiology, Changzheng Hospital, Second Military Medical University, Shanghai, PR China |
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| Abstract: | Hydrogen gas was reported to reduce reactive oxygen species and alleviate cerebral, myocardial and hepatic ischemia/reperfusion (I/R) injuries. This paper studied the effect of hydrogen-rich saline, which was easier for clinical application, on the intestinal I/R injury. Model of intestinal I/R injury was induced in male Sprague-Dawley rats. Physiological saline, hydrogen-rich saline or nitrogen-rich saline (5 ml/kg) was administered via intravenous infusion at 10 min before reperfusion, respectively. The intestine damage was detected microscopically and was assessed by Chiu score system after I/R injury. In addition, serum DAO activity, TNF-α, IL-1β and IL-6 levels, tissue MDA, protein carbonyl and MPO activity were all increased significantly by I/R injury. Hydrogen-rich saline reduced these markers and relieved morphological intestinal injury, while no significant reduction was observed in the nitrogen-rich saline-treated animals. In conclusion, hydrogen-rich saline protected the small intestine against I/R injury, possibly by reduction of inflammation and oxidative stress. |
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| Keywords: | Hydrogen-rich saline intestinal ischemia/reperfusion inflammation oxidative stress |
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