Hydrocaffeic and p-coumaric acids,natural phenolic compounds,inhibit UV-B damage in WKD human conjunctival cells in vitro and rabbit eye in vivo |
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| Authors: | Mar Larrosa Maura Lodovici Lucia Morbidelli Piero Dolara |
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| Institution: | 1. Department of Pharmacology, University of Florence, Viale Pieraccini 6, 50139, Florence, Italymar.larrosa@unifi.it;3. Department of Pharmacology, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy;4. Department of Molecular Biology, University of Siena, Via A. Moro 2, 53100, Siena, Italy |
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| Abstract: | This paper studied the effect on UV-B ocular damage of 10µm hydrocaffeic acid (HCAF) alone and as a mixture (MIX) (5µm HCAF+5µm p-coumaric acid). Since ocular UV-B damage is mediated by reactive oxygen species, the aim was to test if HCAF and MIX could reduce oxidation damage in human conjunctival cells (WKD) in vitro and in cornea and sclera of rabbits in vivo. After UVB irradiation (44 J/m2) of WKD cells, 8-oxodG levels in DNA were markedly increased and this effect was attenuated by HCAF and MIX. Rabbit eyes were treated by application of HCAF and MIX drops before UV-B exposure (79 J/m2). Corneal and scleral DNA oxidation damage, xanthine-oxidase (XO) activity and malondialdehyde levels (MDA) in corneal tissue and prostaglandin E2 (PGE2) in the aqueous humour were reduced by HCAF alone and in combination with p-coumaric acid, showing their potential as a topical treatment against UV-B damage. |
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| Keywords: | UV-B 8-oxo-2′-deoxyguanosine phenolic acids reactive oxygen species XO |
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