Roscovitine inhibits ERK1/2 activation induced by angiotensin II in vascular smooth muscle cells |
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Authors: | Li Ai-Ying Han Mei Zheng Bin Wen Jin-Kun |
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Institution: | Department of Biochemistry and Molecular Biology, Hebei Medical University, Shijiazhuang, China. |
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Abstract: | Roscovitine is a potent CDK inhibitor often used as a biological tool in cell-cycle studies, but its working mechanism and real targets in vascular smooth muscle cells (VSMCs) remain unclear. In this study, we observed that ERK1/2 phosphorylation induced by Ang II was abrogated by pretreating VSMCs with roscovitine for 15h. Pretreating VSMCs with roscovitine also inhibited Ang II-induced c-Jun expression and phosphorylation. We further demonstrated that roscovitine could suppress the DNA binding activity of c-Jun and activation of angiotensinogen promoter by Ang II. These results suggest that roscovitine represses Ang II-induced angiotensinogen expression by inhibiting activation of ERK1/2 and c-Jun. |
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