Effect of temperature on the physiology and cytology of the methylotrophic yeastCandida boidinii growing in methanol-limited chemostat |
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Authors: | O Volfová Z ?i?ka M Anděrová |
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Institution: | (1) Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague 4 |
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Abstract: | All deviations from optimum cultivation temperature affect strongly the physiology and morphology of cells ofCandida boidinii strain 2 during growth in methanol-limited chemostat. The optimum cultivation temperature was 28–30 °C at which maximum cell
concentration and maximum cell yield (Y
S 0.4 g/g) were achieved. At suboptimal growth temperatures the cells were rich in cell protein, RNA, alcohol oxidase (AO)
and in peroxisomes. Formation of cubic peroxisomes and a 20 % decrease of budding cells in the population was observed. At
supraoptimal growth temperatures (>30 °C) a sharp decrease in AO activity was accompanied by degradation of peroxisomes in
the cells. The culture forms pseudomycelium: at 34 °C the cells stop growing and they are washed out of the bioreactor. |
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