Production of haploid plantlets in anther cultures of Albizzia lebbeck L. |
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Authors: | P K Gharyal A Rashid S C Maheshwari |
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Institution: | (1) Department of Botany, University of Delhi, 110007 Delhi, India |
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Abstract: | Anthers of Albizzia lebbeck on B5 medium (BM) supplemented with kinetin (2 mg/l) and 2, 4-D (0.5 mg/l) showed callus initiation from microspores. Differentiation of embryoids and shoots was obtained on BM + BAP (1 mg/l) + IAA (0.5 mg/l) and of roots on BM. Root tip squashes of the regenerated plantlets showed the haploid chromosome number (n=13), confirming the microspore origin of the regenerants. |
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