Analysis of human plasma as an exposure level monitor for carcinogenic tryptophan pyrolysis products |
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| Authors: | S Manabe O Wada |
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| Institution: | Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan. |
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| Abstract: | A high-performance liquid chromatography method for detecting 3-amino-1,4- dimethyl-5H-pyrido4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido4,3-b]indole (Trp-P-2) in human plasma was developed. Plasma samples of 10 normal subjects were examined. Trp-P-1 and Trp-P-2, carcinogenic tryptophan pyrolysis products, were detected in all specimens, and the concentrations of Trp-P-1 and Trp-P-2 in plasma were 68.31 +/- 24.03 fmoles/ml (mean +/- S.D., n = 10) and 18.79 +/- 4.99 fmoles/ml, respectively. Our results suggest that plasma levels of carcinogenic tryptophan pyrolysis products may be useful indicators for estimating the exposure levels of the dietary carcinogens. |
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